Curcumol inhibits encephalomyocarditis virus by promoting IFN-β secretion

Abstract Background Encephalomyocarditis virus (EMCV) infection can cause reproductive failure in sows and acute myocarditis sudden death piglets. It has caused huge economic losses to the global pig industry that is why it necessary develop effective new treatment compounds. Zedoary turmeric oil been used for treating myocarditis. Curcumol extracted from roots of curcuma one main active ingredient zedoary oil. The anti-EMCV activity curcumol along with molecular mechanisms involved a focus on IFN-β signaling pathway was investigated this study. Method 3-(4,5-dimethyithiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay determine maximum non-toxic concentration (MNTC), 50% cytotoxic (CC 50 ), inhibition rate (MIR) (EC ) against EMCV. Through EMCV load, anti-viral effect quantitatively determined using real-time quantitative PCR (qPCR). expression ELISA. Western blot amounts MDA5, MAVS, TANK, IRF3 P-IRF3 proteins human embryonic kidney 293 T (HEK-293 T) cells infected Results results MTT showed compared ribavirin positive control group, ratio greater but selection index (SI) value much smaller than ribavirin. qPCR significantly reduced replication HEK-293 cells. (0.025 mg/mL) increased mRNA EMCV-infected while did not. ELISA mg/mL 0.0125 protein inhibit degradation TANK mediated by promote MDA5 P-IRF3, level MAVS remain unchanged. Conclusion biological which we suggest its mechanisms.